Note
Go to the end to download the full example code.
PMD Compression#
Simple PMD compression, the hello world of masknmf

/home/runner/work/masknmf-toolbox/masknmf-toolbox/masknmf/utils/_cuda.py:10: UserWarning: You've explicitly selected to perform computations on the cpu, performance will be significantly slower
warn(
[25-12-16 00:16:33]: Starting compression
[25-12-16 00:16:33]: sampled from the following regions: [0]
[25-12-16 00:16:33]: We are initializing on a total of 2000 frames
[25-12-16 00:16:33]: Loading data to estimate complete spatial basis
[25-12-16 00:16:33]: skipping the pruning step for frame cutoff
[25-12-16 00:16:33]: Finding spatiotemporal roughness thresholds
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[25-12-16 00:16:38]: Running Blockwise Decompositions
[25-12-16 00:16:38]: Constructed U matrix. Rank of U is 180
[25-12-16 00:16:38]: PMD Objected constructed
/opt/hostedtoolcache/Python/3.12.12/x64/lib/python3.12/site-packages/fastplotlib/graphics/features/_base.py:18: UserWarning: casting float64 array to float32
warn(f"casting {array.dtype} array to float32")
# test_example = true
import masknmf
import torch
import fastplotlib as fpl
from urllib.request import urlretrieve
import tifffile
urlretrieve(
"https://github.com/flatironinstitute/CaImAn/raw/refs/heads/main/example_movies/demoMovie.tif",
"./demo.tif"
)
# always lazy load raw data by memmaping or other methods
data = tifffile.imread("./demo.tif")
block_sizes = [32, 32]
max_components = 20
# it's recommended to use masknmf on a machine with a GPU
# device = "cuda" if torch.cuda.is_available() else "cpu"
device = "cpu"
# number of frames used to estimate the spatial basis in PMD
num_frames_for_spatial_fit = data.shape[0]
# perform PMD
pmd_result = masknmf.compression.pmd_decomposition(
data,
block_sizes,
num_frames_for_spatial_fit,
max_components=max_components,
device=device,
frame_batch_size=1024
)
# get the residual
pmd_residual = masknmf.PMDResidualArray(data, pmd_result)
# view the movies, note that all these array are LAZY evaluated, allowing you to view extremely large datasets!
iw = fpl.ImageWidget(
data=[data, pmd_result, pmd_residual],
names=["raw", "pmd", "residual"],
figure_kwargs={"size": (1000, 340), "shape": (1, 3)},
cmap="gnuplot2",
)
iw.show()
# use the time slider or set the frame index programmatically
iw.current_index = {"t": 1610}
# manually set vmin-vmax to emphasize noise in raw video
# you can also adjust the vmin-vmax using the histogram tool
# reset the vmin-vmax by clicking the buttons under "ImageWidget Controls"
for image in iw.managed_graphics:
image.vmax = 3_200
# remove toolbar to reduce clutter
for subplot in iw.figure:
subplot.toolbar = False
# ignore the remaining lines these are just for docs generation
figure = iw.figure
if __name__ == "__main__":
print(__doc__)
fpl.loop.run()
Total running time of the script: (0 minutes 50.860 seconds)